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A peptide can be 99% pure by HPLC and still carry LAL-detectable endotoxin levels that matter for injection use

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The lipid residue in the LC-MS injection vial was redissolved with 300 μL of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20°C for 2 h