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Through RNAseq of primary NSCs treated with O-GlcNAc transferase inhibitor they identified upregulated transcription factors associated with NSC function, including STAT3 , a key inducer of astrocyte differentiation (He et al., 2005), and they confirmed the critical role of STAT3, since a gliogenic fate shift in NSCs was triggered by loss of O-GlcNAcylation of STAT3 at Threonine 717 (T717) in the hippocampus